19/11/2017 3:21 PM

BVT Technologies, a.s. is very active in the research and development of new types of electrochemical sensors and biosensors. This research and development takes place with the support from the EU Framework Programmes and Czech national projects.

Currently we are participating in 1 project:
  • ACTIVE DOSAGE OF CYTOSTATIC

    CZ.01.1.02/0.0/0.0/15_018/0004617, co-financed by the European Union.

The aim of the project is a research that allows optimal dosing of cytotoxic agents to maintain their optimal effective concentration. The device will be the basis for a system for enabling a controlled dosage of drugs with feedback. The device will detect Doxorubicin and its analogues. The project will produce two prototypes of the device. Prototypes will serve for following research leading to production of instruments for scientific clinical workplaces. The financial resources obtained from the sale of instruments to scientific workplaces will be used to complete clinical trials and obtain a permit to sell the equipment as a standard medical device.

Past projects:
  • INTEGRATED MONITORING SYSTEM - environmental traffic contamination - Technology agency of Czech Republic
  • DLC - TIP programme of Ministry of Industry and Trade of the Czech Republic 
  • InFuLOC - EU 7th Framework Programme - Marie Curie
  • NIMS - Czech Science Foundation
  • DTK - TIP programme of Ministry of Industry and Trade of the Czech Republic
  • EU-CLAMP - EU 7th Framework Programme -Research for SMEs
  • BioMedNano - EU 6th Framework Programme
  • Immunolegio - EU 6th Framework Programme
  • BugCheck - EU 6th Framework Programme
  • IBIS - Ministry of Industry and Trade of the Czech Republic

 

 

 

IBIS - Intelligent biosensor system for environmental detection of pesticides and herbicides
 Starting Date: 05.08.2004, End Date: 31.12.2007, Ministry of Industry and Trade \of The Czech Republic, Contract Number: FT-TA/089, Z 188 
 This IBIS project is focused on the basic research of correlations output signal of organophosphate pesticides and photosynthetic herbicides detecting biosensors and classical analytical methods. It includes the basic research of factors which stimulate the toxicity of organophosphate pesticides and photosynthetic herbicides and the factors which suppress the organophosphate pesticides and photosynthetic herbicides action are to be studied too. The measurement with organophosphate pesticides and photosynthetic herbicides detecting biosensors is standardized. The prototype of analyzer measuring toxicity was developed.   

 

BioMedNANO - Integrating enzymes, mediators and nanostructures to provide bio-powered bio-electrochemical sensing systems

Starting Date: 01.07.2006, End Date: 30.09.2009, Sixth Framework Programme, Contract Number: 017350, Z 433
 

This BIO-MEDNANO STREP-project focuses on research at the frontiers of knowledge of enzymes, mediators, surfaces and immobilisation strategies aimed at improving enzymatic electron transfer reactions for application towards integrated bio-powered biosensing systems for diagnosis and healthcare. Such improvements will be provided by: screening for novel enzymes; modification of enzymes; design of novel nano-structured scaffolds for enzyme immobilisation, to provide devices with improved stability and electron transfer efficiency (sensitivity and/or power output).The long-term innovation of the project is to generate profound, molecular level knowledge on the electron transfer processes taking place between biological molecules and nanostructured electrochemically active materials and further apply this knowledge to integrated bio-powered biosensing systems, which can be exploited in the area of diagnosis and healthcare.
 
IMMUNOLEGIO - Rapid biotechniques based on imunosensors for in-siu detection of legionella in industrial and environmental water samples
Starting Date: 01.10.2006, End Date: 31.03.2009, Sixth Framework Programme, Contract Number: 032169, Z 418
Legionella pneumonia is found in aquatic habitats, especially in potable water systems, cooling tower systems, heat exchanger systems. Human infections occur through the inhalation of aerosols contaminated by Legionella spp. An estimated 8,000 to 18,000 people are stricken with Legionnaires disease in EU-15 alone each year. Up to 30% of people who have Legionnaires disease die. The conventional method for detection of Legionella is a complicated procedure involving isolation in a selective medium. However, this method presents several problems, including the presence of viable but nonculturable pathogens, loss of viability after collection and the long time required for culture and confirmation, which takes several days. In addition, detection is more difficult, since Legionella can be inhibited and masked by the rapid abundant growth of other microorganisms. In order to avoid the problems encountered with the conventional analysis method, an alternative method for rapidly detecting Legionella with high sensitivity is needed. The aim of the proposed project is to develop new bioanalytical instruments in order to arrive at analytical tool for the in-situ detection of Legionella by a rapid and low cost assay. The main objective is to develop a magnetoresistive biosensor device employing magnetic particles as biological markers, which allow for real time measurements in order to achieve a detection limit of 50 CFU L (colony-forming units per liter) . It will ensure that the Immunolegio device passes the strict threshold level regulation fixed at 100 CFU L and enabling a complete analysis in a total test time of 30 minutes, with a minimal enrichment stage.   

 

Bugcheck - A rapid hand-held analyser for control of microorganisms in the complete meat supply chain

Starting Date: 01.07.2006, End Date: 1.04.2008, Sixth Framework Programme, Contract Number: 17969, Z 411

Food borne pathogens in processed ready-to-eat products pose a serious threat to consumers with compromised immune system. Sensitive, specific and rapid detection of such pathogens is thus essential at production level to prevent their entrance into the human food chain.

Conventional microbiological detection methods simply take too long (2 to 7 days) to detect and identify pathogens in food and no real time data is available. Other traditional testing methods, such as ELISA, are also relatively costly and time-consuming. Traditional methods require the taking of a product sample, its posterior culturing until sufficient microorganisms have been generated to enable ready detection on culture plates. While a number of methods such as PCR may provide faster detection (6-12 hours) they involve complex procedures and highly specialized trained personnel.

In today's modern food supply chain, products enter and leave the market within two to three days. Slow traditional analytical methods are clearly deficient as they enable contaminated meat products to reach the market, resulting in human disease and even mortality. Moreover, most analyses need to be carried out in large analytical laboratories as the required instrumentation is expensive and requires highly qualified staff. Only very large farms and slaughterhouses can thus afford to perform regular, on-site, microbiological checks.

There is a clear need for a rapid, handheld, easy to use and cost effective microbiological analyzer which is tailored and well suited to the needs of the meat industry to enable its use on the processing floor thus allowing rapid process control testing. A biosensor based approach presents a promising and sensitive alternative tool for the detection of low numbers of cells in a question of minutes (with no need for enrichment steps) as opposed to days. Electrochemical methods would also enable the application of more user friendly and cheaper instrumentation.
 

NIMS - Nanoimmunosensors for detection of cytokines

Starting Date: 01.01.2007, End Date: 31.12.2012, Czech Science Foundation, Contract No: KAN200520702, Z 463 
This NIMS project aims at developing a concerted research effort towards understanding of basic principles underlying the preparation of rapidly responding, selective, sensitive and robust nanoimmunosensors for detection of biological ligands and especially of cytokines. Four academic groups have joined forces here with three research-intensive industrial partners, in order to develop an expert collaboration aiming on analysis of structure-function relationships of recombinant binding proteins, their immobilization on colloidal nanoparticles and electrode nanoarrays. Nanoarray electrode surface preparation and principles of their integration into biosensors will be studied. The dynamic response range and selectivity characteristics of model immunochromatographic and electrochemical sensors will be evaluated using a model example of interferon gamma detection in defined solutions, as well as in real biological samples derived from blood of individuals suspected of tuberculosis infection.   

 

Infuloc - integrated and functional lab-on-Chip

Starting Date: 01.03.2009, End Date: 28.02.2013
 

Seventh Framework Programme Support for training and carem development of researches (Marie Curie) Industry-Academia Partnership and Pathways (IAPP), Contract No: 230749, Z 791

The objective of this project is to create Lab-on-chip devices that allow sensitive analyte detection as well as being highly integrated and have high functionality. The project will develop lab-on-chip platform technologies for fluidic manipulations and analyte detection and develop specific Lab-on-Chip (LOC) applications. Integrated and easy to use fluidic manipulations within LOC devices will be achieved through firstly use of electro-osmotic flow (EOF) and through on-chip pressure system and passive valves. We will also demonstrate that LOC devices can be designed to perform sophisticated fluidic operations over a large element array of microchambers. A large scale valving integration approach will be used to achieve discrete fluidic operation for each microchamber element within the array. A convenient LOC macro-micro world interface for fluidic, mechanical and electrical elements will be developed as well as sensitive optical and electrochemical strategies for detection. Novel optical detection approach which involves increasing the pathlength of the radiation within the microfluidic channel will be further advanced for highly sensitive optical detection within LOC devices. Sensitive electrochemical transduction will also be developed. The developed LOC platform technologies will be incorporated as part of a disposable cartridge to be inserted within a handheld reader device for bioanalysis. Specifically, we will develop systems for (i) biochemical discharges of airborne enzymes, (ii) continuously monitored bioreactor array and (iii) natural toxic monitor for potatoes. The project will bring together a wide range of leading expertise from the public sector and research organisations to create LOC devices that are reproducible, have a small footprint, are easy to use and demonstrate commercial advantage. The project will lead to long term collaborations between the partners through e.g. the commercialisation of project outcomes.

 

Biolisme - Speedy system for sampling and detecting Listeria monocytogenes in agro-food and related European industries

Starting Date: 01.06.2009, End Date: 31.01.2010, Seventh Framework Programme SP4 - Capacities, Contract Number: 232037, Z 795

 

DLC - Nová generace elektrochemických senzorů a biosenzorů s využitím tenkých modifikovaných DLC vrstev

Starting Date: 01.04.2009, End Date: 30.04.2013, Contract Number: FR-TI1/118

New generation of electrochemical sensors and biosensors using thin modificated DLC layers 

The project aims to develop technology for the production of electrochemical sensors whose working electrode is modified by DLC (diamond-like carbon) layer which contain fluorine, boron or other elements. Such sensors are currently not available on market. The project aims also to develop applications of these sensors. Two specialized devices for DLC layer will be developed in project.

 

DTK - Nové elektrochemické senzory pro detekci těžkých kovů

Starting Date: 01.12.2009, End Date: 30.06.2011, Contract Number: FR-TI1/076
   Program research project will cover the new electrochemical methods for detection of heavy metals, based on the parallel measurement of samples and standards. The evaluation unit and software will be designed for the detection of heavy metals using a new electrochemical sensor with microreactor. Research and development of new electrochemical sensors use and develop technology applied by BVT Technologies, as a patent in 2009 (PV 2009-22). Evaluation signals of stripping analysis will be dealt with synchronous detection. 

EUCLAMP

Starting Date: 01.12.2010, End Date: 30.11.2012, Contract Number: FP7-SME-2010-1

The project began in 2010. The project will develop the connection of Probe microEye with BVT flow cell FC2. The result will be used for continuous monitoring of diabetes patient.

Europe faces a diabetes epidemic. More than 55 million people in Europe are currently diagnosed with diabetes and with an estimated 20% increase by 2030, the disease is certain to stay one of the most challenging health problems this century. Especially as diabetes no longer is a disease exclusively for adults, but affects children, young people and adults of all ages. Despite the high prevalence of diabetes, the choice of anti-diabetic drugs is still limited and two thirds of patients with diabetes do not achieve the recommended glycaemic target levels. For each new anti-diabetic drug, it is essential to investigate the metabolic effect over time. The glucose clamp technique is regarded as the gold standard to evaluate the effectiveness of new anti-diabetic drugs. There are however only a few centres with limited research capacities that have experience in using the clamp technique, because automated clamp devices are no longer commercially available and the existing techniques are confounded by a number of limitations. Increased clamp capacities are urgently needed for the development of new, more efficacious anti-diabetic drugs.

Through the integration of newly emerging technologies proposed in part by the 4 participating SME organisations and with the outsourced research capacity of 3 of Europe s leading RTD performers, the EU-CLAMP project aims to develop a new generation automated clamp device that will overcome the limitations of the existing devices by incorporating micro-dialysis technique for reliable continuous glucose monitoring without blood loss. The EU-CLAMP project will facilitate clamp testing in a more efficient and cost effective manner. In addition to the significant contribution that will be made to development of improved treatment options for diabetes, the project will provide a platform from which the competitiveness of the participating SMEs can be improved offering alignment to the needs of their long term business strategies.
For more information please contact
 

Lucie Ježová

Eng. Lucie Ježová
Consultancy department
E-mail: consultancy (at) bvt.cz
Tel.: + 420 563 034 298+ 420 563 034 298